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The NDI stream created does not contain any alpha-channel/transparency and the Video Settings in OBS Studio will be locked when one of the NDI outputs are enabled, as NDI will use the same Output (Scaled) Resolution and Frame Rate set by you there. In the NDI Output Settings dialog you can give the NDI stream a name to properly identify the NDI stream on the network if your have created multiple.
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The first method is to create an NDI stream of the main (stream) output and/or the preview output (studio mode) in OBS Studio containing all sources visible and the mixed final audio stream, by enabling the NDI Main and/or Preview Output via Tools → NDI Output Settings.
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If you are installing this plugin without having the NDI Redistributable installed first, make sure to reboot your computer after installing the plugin, as it will automatically install this for you. You can use OBS Studio on a computer to capture a game and other sources and send this out as an NDI stream with the OBS Studio NDI Plugin by Palakis. For information how to receive NDI streams in Streamlabs Desktop see this article. This article is for setting up NDI streams to receive in Streamlabs Desktop or other application.
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